Journal: Food Science & Nutrition

Article Title: Formononetin, a Key Component of Danggui Buxue Decoction, Inhibits Metastasis of Triple‐Negative Breast Cancer by Modulating SNAI2‐Driven EMT

doi: 10.1002/fsn3.71286

Figure Lengend Snippet: Network pharmacology analysis of key interactions. (A) Venn diagram illustrating the shared targets of the Formononetin and TNBC. (B) The identification of key proteins was based on a PPI network. (C) Cluster analysis of the core gene SNAI2. (D) Construction of a network to illustrate the interactions between the Formononetin–TNBC targets. (E) GO and KEGG enrichment analysis.

Article Snippet: The membranes were incubated with primary antibodies SNAI2 (12129‐1‐AP, 1:5000, Proteintech), E‐Cadherin (20874‐1‐AP, 1:20000, Proteintech), α‐SMA (14395‐1‐AP, 1:1000, Proteintech), Collagen I (ab138492, 1:100, Abcam), Fibronectin (15613‐1‐AP, 1:200, Proteintech), and β‐actin ( AWA80001 , 1:500, Abiowell) overnight at 4°C.

Techniques:

Journal: Food Science & Nutrition

Article Title: Formononetin, a Key Component of Danggui Buxue Decoction, Inhibits Metastasis of Triple‐Negative Breast Cancer by Modulating SNAI2‐Driven EMT

doi: 10.1002/fsn3.71286

Figure Lengend Snippet: Formononetin targeting SNAI2. (A) Molecular docking analysis was used to predict the binding of the active ingredient Formononetin to the core gene SNAI2. (B) The binding of Formononetin to SNAI2 was confirmed by the DARTS method. Data are presented as mean ± SD, n = 3. * p < 0.05 vs. the Control group (0 μm/mL Formononetin + 0 μg/mL Pronase), # p < 0.05 vs. the Pronase group. Control group: Not treated with Pronase and Formononetin. Pronase group: Only treated with Pronase.

Article Snippet: The membranes were incubated with primary antibodies SNAI2 (12129‐1‐AP, 1:5000, Proteintech), E‐Cadherin (20874‐1‐AP, 1:20000, Proteintech), α‐SMA (14395‐1‐AP, 1:1000, Proteintech), Collagen I (ab138492, 1:100, Abcam), Fibronectin (15613‐1‐AP, 1:200, Proteintech), and β‐actin ( AWA80001 , 1:500, Abiowell) overnight at 4°C.

Techniques: Binding Assay, Control

Journal: Food Science & Nutrition

Article Title: Formononetin, a Key Component of Danggui Buxue Decoction, Inhibits Metastasis of Triple‐Negative Breast Cancer by Modulating SNAI2‐Driven EMT

doi: 10.1002/fsn3.71286

Figure Lengend Snippet: Formononetin inhibits cell proliferation, invasion, migration, and EMT by regulating SNAI2. (A) The effects of DBD and Formononetin on SNAI2 protein expression were detected by Western Blot. (B) The overexpression efficiency of oe‐SNAI2 was verified by qRT‐PCR. (C) The level of SNAI2 protein was detected by Western Blot. (D) Cell viability of TNBC cells was assessed by CCK‐8. (E) The migration of TNBC cells was assessed by the wound healing assay. (F) The invasion of TNBC cells was assessed by Transwell assay. (G) Expression of E‐cadherin, a‐SMA, Collagen I, and Fibronectin. Data are presented as mean ± SD, n = 3. * p < 0.05 vs. the Control group. # p < 0.05 vs. the Formononetin + oe‐NC group. Control group: untreated TNBC cells. DBD group: TNBC cells were treated with DBD. Formononetin group: TNBC cells were treated with Formononetin. Formononetin + oe‐NC group: TNBC cells were treated with Formononetin and then transfected with oe‐NC. Formononetin + oe‐SNAI2 group: TNBC cells were treated with Formononetin and then transfected with oe‐SNAI2.

Article Snippet: The membranes were incubated with primary antibodies SNAI2 (12129‐1‐AP, 1:5000, Proteintech), E‐Cadherin (20874‐1‐AP, 1:20000, Proteintech), α‐SMA (14395‐1‐AP, 1:1000, Proteintech), Collagen I (ab138492, 1:100, Abcam), Fibronectin (15613‐1‐AP, 1:200, Proteintech), and β‐actin ( AWA80001 , 1:500, Abiowell) overnight at 4°C.

Techniques: Migration, Expressing, Western Blot, Over Expression, Quantitative RT-PCR, CCK-8 Assay, Wound Healing Assay, Transwell Assay, Control, Transfection